Cat. # | Size | Price | Inventory |
---|---|---|---|
7561S | 100 µl (50 tests) |
REACTIVITY | H R Mk Z B Pg |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Immunofluorescence (Immunocytochemistry) | 1:200 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 |
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted December 2010
Protocol Id: 221
All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit (Methanol) #13593, or individually using the catalog numbers listed below.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
NOTE: Antibodies targeting CD markers or other extracellular proteins may be added prior to fixation if the epitope is disrupted by formaldehyde and/or methanol. The antibodies will remain bound to the target of interest during the fixation and permeabilization process. However, note that some fluorophores (including PE and APC) are damaged by methanol and thus should not be added prior to permeabilization. Conduct a small-scale experiment if you are unsure.
NOTE: Count cells using a hemocytometer or alternative method.
posted July 2009
revised June 2020
实验步骤编号:407
人, 大鼠, 猴, 斑马鱼 , 牛 , 猪
使用与人 COX IV 蛋白中 Lys29 周围残基相对应的合成肽,对动物进行免疫接种来产生单克隆抗体。
细胞色素 C 氧化酶 (COX) 是一种异质寡聚酶,由位于线粒体内膜的 13 个亚基组成 (1-3)。它是呼吸链中的末端酶复合体,可催化分子氧还原成水、线粒体内的质子跨越内膜以合成 ATP 的偶联反应。组成催化核心的 3 个最大亚基通过线粒体 DNA 编码,而其他较小亚基(包括 COX IV)则通过细胞核编码。研究表明,COX 活性缺乏与许多人疾病有关 (4)。在细胞研究试验中,COX IV antibody 可有效用作线粒体上样对照。
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