Cell Signaling Technology

Product Pathways - Adhesion

Mesothelin (D9R5G) XP® Rabbit mAb #99966

mesothelioma  

No. Size Price
99966S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
99966T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
99966 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 46-48, 70 Rabbit IgG
IHC-P 1:250

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Mesothelin (D9R5G) XP® Rabbit mAb recognizes endogenous levels of total mesothelin protein. The antibody detects both uncleaved (full-length) and cleaved forms of mesothelin, but does not detect the cleaved fragment corresponding to megakaryocyte-potentiating factor (MPF).

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human mesothelin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Mesothelin (D9R5G) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded OVCAR8 (left) and PC3 (right) cell pellets using Mesothelin (D9R5G) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Mesothelin (D9R5G) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Mesothelin (D9R5G) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Mesothelin (D9R5G) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from OVCAR8 cells, untreated (-) or PNGase F-treated (+), using Mesothelin (D9R5G) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

The MSLN gene encodes a 69 kDa precursor protein that is proteolytically cleaved to yield Megakaryocyte Potentiating Factor (MPF) and a GPI-anchored membrane protein termed mesothelin (1). Expression of (cleaved) mesothelin is largely confined to mesothelial cells of normal pleura, pericardium, and peritoneum, but has been reported to be overexpressed in some cancers, including mesothelioma, and some pancreatic and ovarian adenocarcinomas (1,2). Although suggested to be involved in cell adhesion, the physiological functions of mesothelin have not been determined. It is known, however, that mesothelin can be shed from the cell surface following cleavage by TNF-α converting enzyme. Research studies show that serum levels of mesothelin are markedly increased in patients with mesothelioma and ovarian cancer (1), suggesting that serum mesothelin levels may have utility as a cancer biomarker (1-3).

  1. Pastan, I. and Hassan, R. (2014) Cancer Res 74, 2907-12.
  2. Villena-Vargas, J. and Adusumilli, P.S. (2012) Ann Cardiothorac Surg 1, 466-71.
  3. Hassan, R. et al. (2004) Clin Cancer Res 10, 3937-42.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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