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9996
Oligomycin
激活剂与抑制剂
化学调节物

Oligomycin #9996

Citations (0)
Western blot analysis of extracts from 293 cells, untreated (-) or treated with Oligomycin (5 µM, 30 min; +), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 (upper) or AMPKα Antibody #2532 (lower).

Product Usage Information

Oligomycin is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 5 mg in 1.26 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 0.5 - 10 μM for 0.5 - 8 hours.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 791.1 g/mol
Purity 97%
Molecular Formula Isomer A: C45H74O11 Isomer B: C45H72O12 Isomer C: C45H74O10
CAS 1404-19-9
Solubility Soluble in DMSO at 300mg/ml and EtOH at 200mg/ml.

Background

During oxidative phosphorylation in mitochondria, the electron transport system pumps H+ from the matrix into the intermembrane compartment. This process generates a H+ gradient across the mitochondrial inner membrane. This H+ gradient is used to drive mitochondrial FoF1 ATPase to convert ADP to ATP. Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 9996L
Cat. # Size Price Inventory
9996L
5 mg