Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

FoxO3a (D7D3Y) Mouse mAb #99199

No. Size Price
99199S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
99199 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 82 to 97 Mouse IgG1
IP 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

FoxO3a (D7D3Y) Mouse mAb detects exogenous and endogenous levels of total FoxO3a protein. The antibody does not detect the exogenously expressed family members FoxO1 or FoxO4.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly381 of human FoxO3a.

IF-IC

IF-IC

Confocal immunofluorescent analysis of PC-3 cells, serum-starved overnight (left) or serum-starved overnight and treated with LY294002 #9901 (50 μM, 1 hr; right), using FoxO3a (D7D3Y) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, MRK-nu-1, and PC-3 cells using FoxO3a (D7D3Y) Mouse mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).

IP

IP

Immunoprecipitation of FoxO3a from 293T cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb Isotype Control #5415, and lane 3 is FoxO3a (D7D3Y) Mouse mAb. Western blot was performed using FoxO3a (D7D3Y) Mouse mAb.

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

  1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
  2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
  3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
  4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
  5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
  6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
  7. Seoane, J. et al. (2004) Cell 117, 211-23.
  8. Arden, K.C. (2004) Mol Cell 14, 416-8.
  9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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