Cell Signaling Technology

Product Pathways - Metabolism

NMNAT1 (D7O4N) Rabbit mAb #98354

No. Size Price
98354S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
98354 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 29 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

NMNAT1 (D7O4N) Rabbit mAb recognizes endogenous levels of total NMNAT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding A268 of human NMNAT1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T, HeLa, and SK-MEL-28 using NMNAT1 (D7O4N) Rabbit mAb (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower).

Background

Nicotinamide mononucleotide adenylyl transferases (NMNATs) catalyze the reversible reaction of ATP with NaMN (nicotinic acid mononucleotide) or NMN (nicotinamide mononucleotide) to produce NaAD (nicotinic acid adenine dinucleotide) or NAD (nicotinamide adenine dinucleotide). NAD is an essential cofactor or substrates for many enzymes like PARP1 and Sirt1 that regulate diverse cellular processes including oxidative reactions and transcription. NMNATs maintain NAD levels for internal homeostasis (1,2). NMNAT1 is localized to the nucleus and loss-of-function mutant in mice causes embryonic lethality (3). In humans, several different NMNAT1 mutations are associated with Leber congenital amaurosis (LCA), the most common cause of inherited childhood blindness (4-7).

  1. Jayaram, H.N. et al. (2011) Curr Med Chem 18, 1962-72.
  2. Ali, Y.O. et al. (2013) Trends Neurosci 36, 632-40.
  3. Conforti, L. et al. (2011) FEBS J 278, 2666-79.
  4. Chiang, P.W. et al. (2012) Nat Genet 44, 972-4.
  5. Koenekoop, R.K. et al. (2012) Nat Genet 44, 1035-9.
  6. Perrault, I. et al. (2012) Nat Genet 44, 975-7.
  7. Falk, M.J. et al. (2012) Nat Genet 44, 1040-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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