Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-eIF2α (Ser51) Antibody #9721

eif   eif-2   eif2-alpha   eif2a   eif2alpha   elf-2   elf2  

No. Size Price
9721L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
9721S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9721 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 38 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites.

Phospho-eIF2alpha (Ser51) Antibody检测仅在Ser51位点磷酸化的内源性eIF2alpha蛋白水平。该抗体不与其它位点磷酸化的elF2alpha蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha Antibody #9722 (lower).

使用Phospho-eIF2alpha (Ser51) Antibody (上图)和eIF2alpha Antibody #9722 (下图),免疫印迹(Western Blot)分析在PC12细胞系中Phospho-eIF2α (Ser51)和eIF2alpha蛋白水平水平,细胞分为对照组和thapsigargin(300 nM)处理组。


Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

研究证明在不同的应激条件下真核生物起始因子2 (eIF2) α亚型的磷酸化可下调蛋白质的合成。eIF2蛋白与GTP以及起始tRNA(Met-tRNAi)结合,然后将Met-tRNA转移到40S核糖体亚基上共同形成43S前起始复合物(1,2)。eIF2通过eIF2B催化反应将GTP交换GDP从而促进新一轮翻译的起始(1,2)。病毒感染(PKR)、内质网应激(endoplasmic reticulum stress)(PERK/PEK)、氨基酸饥饿(GCN2)或血红素缺乏症(HRI)能够激活相关的激酶,这些激酶可以使eIF2的α亚基磷酸化(3,4)。这个磷酸化稳固eIF2-GDP-eIF2B复合物,同时期抑制eIF2B的降解。通过IFN-γ 和TNF-α的诱导的PKR能有效的诱导eIF2α蛋白在Ser51位点磷酸化(5,6)。

  1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
  2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
  3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
  4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
  5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
  6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :