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Acetyl-β-Catenin (Lys49) Antibody #9534

No. Size Price
9534S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9534 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 92 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Pig,

Specificity / Sensitivity

Acetyl-β-Catenin (Lys49) Antibody detects endogenous β-catenin only when acetylated at Lys49.

Acetyl-β-Catenin (Lys49) 抗体仅能识别49位赖氨酸被乙酰化后的内源性β-catenin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin. Antibodies were purified by protein A and peptide affinity chromatography.

合成与人β-catenin 49位赖氨酸乙酰化及华人邻近氨基酸残基序列一致的乙酰化多肽,免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from 293 cells, untransfected or transiently transfected with a construct expressing CBP, using Acetyl-β-Catenin (Lys49) Antibody (Top), or total β-Catenin Antibody (Amino-terminal Antigen) #9581 (Bottom).

使用Acetyl-β-Catenin (Lys49) 抗体 (上),或 total β-Catenin抗体(氨基末端抗原) #9581 (底)对未转染或瞬转CBP表达质粒的293细胞提取物进行western blot实验。


β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin and is one of few residues frequently mutated in thyroid anaplastic carcinoma (9). CBP (CREB-binding protein) binds and acetylates β-catenin at Lys49 (10, 11).


  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Polakis, P. (2000) Genes Dev. 14, 1837-5181.
  10. Takemaru, K.I. and Moon, R.T. (2000) J. Cell Biol. 149, 249-254.
  11. Wolf, D. et al. (2002) J. Biol. Chem. 277, 25562-25567.

Application References

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