Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb #94994

No. Size Price
94994S 100 µl ( 10 western blots ) ¥4,180.00 现货查询 购买询价 防伪查询
94994T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价 防伪查询
94994 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 86 Rabbit IgG
IP 1:200
F 1:200
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser727.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-431 (left, center; positive) and PC-3 (right; negative) cells, serum-starved and treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 30 minutes; left, right) or Wortmannin #9915 (1 uM) and U0126 #9903 (10 uM, 1.5 hours; center) using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor #8916 (hEGF; 100ng/ml, 30 min; +) and λ-Phosphatase (λPPase; +) using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb (upper) or total Stat3 (D3Z2G) Rabbit mAb #12640 (middle) or β-Actin (D6A8) Rabbit mAb #8457 (lower). PC-3 cells, which do not express Stat3, was used as a negative control.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved U266 cells, untreated (-) or treated with Human Interferon-α1 #8927 (hIFN-α1; 50ng/ml, 15 min; +), Hela cells, untreated (-) or treated with Human Tumor Necrosis Factor-α #8902 (hTNF-α; 20ng/ml, 30 min; +), HT-29 cells growing asynchronously (-) or arrested in mitosis by treatment with Thymidine (2mM, 17 hours) followed by Nocodazole #2190 (100ng/ml, 24 hrs; +), and NIH/3T3 cells, untreated (-) or treated with Human Platelet Derived Growth Factor-AA #8913 (hPDGF-AA; 50ng/ml, 30min; +) using Phospho-Stat3 (Ser727) (D8C2Z) Rabbit mAb (upper) or total Stat3 (124H6) Mouse mAb #9139 (lower).

IP

IP

Immunoprecipitation of Phospho-Stat3 (Ser727) from A-431 cells treated with Human Epidermal Growth Factor #8916 (100ng/ml 30min). Lane1 is 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb. Western blot was performed using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells, untreated (blue) or TPA-treated #4174 (40nM, 15 minutes; green), using Phospho-Stat3 (Ser727) (D8C2Z) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications