Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb #9456

4E-BP-1   4E-BP1   4ebp   BP1   P-4E-BP1   phas   phas-1   Phospho-4EBP1   sc-12884  

No. Size Price
9456S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9456 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 15-20 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb detects endogenous levels of 4E-BP1 when phosphorylated at Ser65.

Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb兔单抗检测仅在Ser65位点磷酸化的内源性4E-BP1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of mouse 4E-BP1.

通过人工合成小鼠4E-BP1 蛋白Ser65位点附近序列的多肽片段来免疫动物从而制备单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, lambda phosphatase treated, or treated with 20% FBS, using Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb (upper) or 4E-BP1 Antibody #9452 (lower).

使用Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb (上图)和4E-BP1 Antibody #9452 (下图),免疫印迹(Western Blot)分析在293细胞系裂解物。


Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

翻译抑制蛋白4E-BP1 (又称 PHAS-1)通过结合翻译起始因子eIF4E蛋白从而抑制帽子结构依赖的翻译。4E-BP1的过磷酸化干扰上述结合并且导致激活帽子结构依赖的翻译(1)。PI3 kinase/Akt通路和FRAP/mTOR激酶通路都调节4E-BP1蛋白的激活(2,3)。在体内4E-BP1多个位点残基被磷酸化(4)。通过FRAP/mTOR蛋白使4E-BP1在Thr37和Thr46位点磷酸化不能阻止它eIF4E结合,但这被认为是为随后在4E-BP1的Ser65和Thr70位点磷酸化进行准备(5)。

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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