Cell Signaling Technology

Product Pathways - Development

Jagged1 (D4Y1R) XP® Rabbit mAb (PE Conjugate) #94449

No. Size Price
94449S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
94449 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Monkey, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Rat, Hamster,

Specificity / Sensitivity

Jagged1 (D4Y1R) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Jagged1 protein. This antibody does not cross-react with Jagged2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1131 of human Jagged1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Jagged1 (D4Y1R) Rabbit mAb #70109.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells (blue) and MKN45 cells (green) using Jagged1 (D4Y1R) XP® Rabbit mAb (PE Conjugate).

Background

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).

  1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
  2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
  3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
  4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
  5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
  6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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