Cell Signaling Technology

Product Pathways - Development

NLK (D9X3C) Rabbit mAb #94350

No. Size Price
94350S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
94350 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 58 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

NLK (D9X3C) Rabbit mAb recognizes endogenous levels of total NLK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NLK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SW480, HCT 116 and C2C12 cells using NLK (D9X3C) Rabbit mAb.

Background

Nemo-like kinase (NLK ) is a serine/threonine-protein kinase that regulates multiple signaling pathways, including Wnt/β-catenin, TGFβ, IL-6, and Notch (1-4). NLK contributes to cell proliferation, differentiation, cell fate determination during early embryogenesis and nervous system development in vertebrates (5-7). Recent studies showed that NLK is aberrantly expressed in various types of cancer where it regulates cancer cell proliferation, migration, invasion and survival (8-11). NLK is localized predominantly in nucleus and at a lower level in cytoplasm(12). Homodimerization of NLK is required for its activation and nuclear localization. NLK is activated via intermolecular autophosphorylation at Thr286 (13). NLK interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4, and LEF-1/TCF (14-18). NLK also associates with E3 ubiquitin ligase NARF and Raptor and regulates their function (19,20).

  1. Smit, L. et al. (2004) J Biol Chem 279, 17232-40.
  2. Ohkawara, B. et al. (2004) Genes Dev 18, 381-6.
  3. Kojima, H. et al. (2005) Proc Natl Acad Sci U S A 102, 4524-9.
  4. Ishitani, T. et al. (2010) Nat Cell Biol 12, 278-85.
  5. Hyodo-Miura, J. et al. (2002) Genes Cells 7, 487-96.
  6. Thorpe, C.J. and Moon, R.T. (2004) Development 131, 2899-909.
  7. Satoh, K. et al. (2007) Mol Cell Biol 27, 7623-30.
  8. Li, M. et al. (2013) Tumour Biol 34, 3995-4000.
  9. Lv, L. et al. (2014) J Cell Biochem 115, 81-92.
  10. Huang, Y. et al. (2013) PLoS One 8, e69148.
  11. Dong, J.R. et al. (2013) Asian Pac J Cancer Prev 14, 7137-41.
  12. Brott, B.K. et al. (1998) Proc Natl Acad Sci U S A 95, 963-8.
  13. Ishitani, S. et al. (2011) Mol Biol Cell 22, 266-77.
  14. Ishitani, T. et al. (2003) Mol Cell Biol 23, 1379-89.
  15. Kanei-Ishii, C. et al. (2004) Genes Dev 18, 816-29.
  16. Kim, S. et al. (2010) J Biol Chem 285, 8122-9.
  17. Togi, S. et al. (2011) J Biol Chem 286, 19170-7.
  18. Szypowska, A.A. et al. (2011) Antioxid Redox Signal 14, 563-78.
  19. Yamada, M. et al. (2006) J Biol Chem 281, 20749-60.
  20. Yuan, H.X. et al. (2015) Genes Dev 29, 2362-76.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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