Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) #9429


No. Size Price
9429S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9429T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
9429 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 18, 43 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone.

Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) 抗体能够检测内源性水平的小鼠和大鼠 Asp387剪切的caspase-8。该抗体能够检测剪切产物包括含有P18(p43)亚单位的前体结构域,也可以检测独立的P18亚单位。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to the Asp387 cleavage site of rodent caspase-8. Antibodies were purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, mock transfected (-) or transfected with a construct overexpressing mouse caspase-8 (+), using Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by TNF-α #8902 (20 ng/ml, 4 hours), using Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific).


Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8,并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases,如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化,包括DNA的片段化和细胞固缩。

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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