Cell Signaling Technology

Product Pathways - Metabolism

AHCYL1/IRBIT (D3A5G) Rabbit mAb #94248

adenosylhomocysteinase-like 1   AdoHcyase 2   AHCYL1   DC-expressed AHCY-like molecule   DCAL   dendritic cell expressed AHCY-like protein   inositol 1.4.5-trisphosphate receptor-binding protein   IRBIT   PRO0233   Putative adenosylhomocysteinase 2   S-adenosyl homocysteine hydrolase homolog   S-adenosyl-L-homocysteine hydrolase 2   S-adenosylhomocysteine hydrolase-like 1   S-adenosylhomocysteine hydrolase-like protein 1   SAHH2   XPVKONA  

No. Size Price
94248S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
94248 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 61 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

AHCYL1/IRBIT (D3A5G) Rabbit mAb recognizes endogenous levels of total AHCYL1/IRBIT protein. This antibody does not cross-react with AHCYL2/IRBIT2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu50 of human AHCYL1/IRBIT protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using AHCYL1/IRBIT (D3A5G) Rabbit mAb.

Background

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) is a member of S-adenosylhomocysteine hydrolase family, which participates in the metabolism of S-adenosyl-L-homocysteine (1). Two Drosophila homologs of S-adenosylhomocysteine hydrolase-like proteins, dAhcyL1 and dAhcyL2, were identified as novel components of methionine metabolism (2). dAhcyL1 and dAhcyL2 function as dominant-negative regulators of S-adenosylhomocysteine hydrolase (2). Global down-regulation of both dAhcyL1 and dAhcyL2 extended life span (2). In addition, brain-specific down regulation of dAhcyL1 extended life span (2). AHCYL1 is also known as inositol 1,4,5-trisphosphate receptor (IP3R) binding protein released with IP3 (IRBIT) (1, 3). This protein binds to the endoplasmic reticulum calcium release channel IP3R and represses its acitivity (1, 3). As a multifunctional regulator, AHCYL1/IRBIT can also form a complex with and suppress the activity of ribonucleotide reductase, thereby influencing the balance of deoxynucleotide triphosphates essential for DNA replication and genomic integrity (4).

  1. Jeong, W. et al. (2012) PLoS One 7, e49204.
  2. Parkhitko, A.A. et al. (2016) Genes Dev 30, 1409-22.
  3. Kawaai, K. et al. (2015) Proc Natl Acad Sci U S A 112, 5515-20.
  4. Arnaoutov, A. and Dasso, M. (2014) Science 345, 1512-5.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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