Cell Signaling Technology

Product Pathways - Metabolism

Arginase-1 (D4E3M™) XP® Rabbit mAb #93668

ARG1   ARGI1   arginase 1   Arginase-1   arginase. liver   autostainer   Bond   Leica   Liver-type arginase   Rx   sc-18351   sc-18354   sc-20150   Type I arginase  

No. Size Price
93668S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
93668T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
93668 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 40 Rabbit IgG
IHC-P 1:100
IF-F 1:50
IF-IC 1:50
IHC-Bond 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IHC-Bond=IHC-Leica® Bond™,

Specificity / Sensitivity

Arginase-1 (D4E3M™) XP® Rabbit mAb recognizes endogenous levels of total arginase-1 protein. This antibody does not cross-react with arginase-2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human liver using Arginase-1 (D4E3M™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat liver using Arginase-1 (D4E3M™) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of mouse liver (positive; left) or small intestine (negative; right) using Arginase-1 (D4E3M™) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) using Arginase-1 (D4E3M™) XP® Rabbit mAb (green). BMDMs were differentiated with M-CSF (20 ng/ml, 7 days) and activated with either IL-4/cAMP (20 ng/ml, 0.5 mM, 24 hours; left) or LPS/IFNγ (50 ng/ml, 20 ng/ml, 24 hours; right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse liver using Arginase-1 (D4E3M™) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse liver and mouse small intestine using Arginase-1 (D4E3M™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-Leica Bond

IHC-Leica Bond

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Background

L-arginine plays a critical role in regulating the immune system (1-3). In inflammation, cancer and certain other pathological conditions, myeloid cell differentiation is inhibited leading to a heterogeneous population of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs) (4). MDSCs are recruited to sites of cancer-associated inflammation and express high levels of arginase-1 (4). Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine and urea (5). Thus MDSCs increase the catabolism of L-arginine resulting in L-arginine depletion in the inflammatory microenvironment of cancer (4, 6). The reduced availability of L-arginine suppresses T-cell proliferation and function and thus contributes to tumor progression (4, 6). Arginase-1 is of great interest to researchers looking for a therapeutic target to inhibit the function of MDSCs in the context of cancer immunotherapy (7). In addition, research studies have demonstrated that Arginase-1 distinguishes primary hepatocellular carcinoma (HCC) from metastatic tumors in the liver, indicating its value as a potential biomarker in the diagnosis of HCC (8, 9).

  1. Albina, J.E. et al. (1989) J Exp Med 169, 1021-9.
  2. Mills, C.D. (2001) Crit Rev Immunol 21, 399-425.
  3. Rodriguez, P.C. et al. (2004) Cancer Res 64, 5839-49.
  4. Gabrilovich, D.I. and Nagaraj, S. (2009) Nat Rev Immunol 9, 162-74.
  5. Wu, G. and Morris, S.M. (1998) Biochem J 336 ( Pt 1), 1-17.
  6. Raber, P. et al. (2012) Immunol Invest 41, 614-34.
  7. Wesolowski, R. et al. (2013) J Immunother Cancer 1, 10.
  8. Sang, W. et al. (2015) Tumour Biol 36, 3881-6.
  9. Geramizadeh, B. and Seirfar, N. (2015) Hepat Mon 15, e30336.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

D4E3M is a trademark of Cell Signaling Technology. Inc.

LEICA is a registered trade​mark of Leica Microsystems IR GmbH.

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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