Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

MTSS1 (D2H4L) XP® Rabbit mAb #93065

No. Size Price
93065S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
93065T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
93065 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 95-105 Rabbit IgG
IP 1:200
IHC-P 1:200
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

MTSS1 (D2H4L) XP® Rabbit mAb recognizes endogenous levels of total MTSS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val650 of human MTSS1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MTSS1 (D2H4L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). U-2 OS and HeLa cells are negative for MTSS1 expression, confirming specificity of the antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NCI-H2052 (left), TT (center), and U20S (right) cells using MTSS1 (D2H4L) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded TT (left) and U-2 OS (right) cell pellets using MTSS1 (D2H4L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using MTSS1 (D2H4L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoid tissue using MTSS1 (D2H4L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MTSS1 (D2H4L) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

IP

IP

Immunoprecipitation of MTSS1 protein from NCI-H2052 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MTSS1 (D2H4L) XP® Rabbit mAb. Western blot analysis was performed using MTSS1 (D2H4L) XP® Rabbit mAb.

Background

Metastasis suppressor 1 (MTSS1) is a multi-functional scaffold protein that was initially discovered using a differential display technique that identified proteins missing from bladder cancer cell lines (1,2). MTSS1 (also known as Missing in Metastasis or MIM) is a cytoskeletal remodeling protein that contains a C-terminal WH2 actin-binding motif (1,3). Presence of an IMD (IRSp53/MIM homology) domain allows MTSS1 to induce F-actin bundling and filopodia formation in cells (4). MTSS1 binds to and activates Rac, a protein known to promote the formation of filopodia and lamellipodia (5). The receptor tyrosine phosphatase δ (PTPRD) is associated with MTSS1 and is required for MTSS1-dependent cytoskeletal change (6,7). MTSS1 is a SHH responsive gene that can help regulate GLI-dependent transcriptional activity (8).

  1. Lee, Y.G. et al. Neoplasia 4, 291-4.
  2. Machesky, L.M. and Johnston, S.A. (2007) J Mol Med 85, 569-76.
  3. Mattila, P.K. et al. (2003) J Biol Chem 278, 8452-9.
  4. Yamagishi, A. et al. (2004) J Biol Chem 279, 14929-36.
  5. Bompard, G. et al. (2005) J Cell Sci 118, 5393-403.
  6. Woodings, J.A. et al. (2003) Biochem J 371, 463-71.
  7. Gonzalez-Quevedo, R. et al. (2005) J Cell Biol 168, 453-63.
  8. Callahan, C.A. et al. (2004) Genes Dev 18, 2724-9.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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