Cell Signaling Technology

Product Pathways - Transcription Factors

NKX3.1 (D6D2Z) XP® Rabbit mAb #92998

Prostate carcinoma  

No. Size Price
92998S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
92998T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
92998 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 30 Rabbit IgG
IHC-P 1:250

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

NKX3.1 (D6D2Z) XP® Rabbit mAb recognizes endogenous levels of total NKX3.1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NKX3.1 protein. The epitope is near the amino terminus, in a region that is 100% conserved between isoforms 1 and 5 of human NKX3.1.

Western Blotting

Western Blotting

Western blot analysis of extracts from human prostate tissue and various cell lines using NKX3.1 (D6D2Z) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). DND-41 is negative for NKX3.1 expression, confirming specificity of the antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP (left) and PC-3 (right) cell pellets using NKX3.1 (D6D2Z) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using NKX3.1 (D6D2Z) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human prostate using NKX3.1 (D6D2Z) XP® Rabbit mAb.

Background

NKX3.1 is a homeobox transcription factor that in mammals plays a defining role in embryonic prostate morphogenesis. The expression of mammalian NKX3.1 is androgen-dependent, restricted primarily to developing and mature prostate epithelium, and is frequently reduced or lost in prostate cancer (1-3). The human NKX3.1 gene is located on chromsome 8p21.2, within a region that shows loss of heterozygosity (LOH) in >50% of prostate cancer cases (2). Allelic loss at the NKX3.1 locus is also common in high grade Prostate Intraepithelial Neoplasia (PIN), thought to be a putative precursor lesion to invasive prostate adenocarcinomas, suggesting that LOH at the NKX3.1 locus is a critical early step in prostate cancer development (4). Notably, the remaining NKX3.1 allele is intact in the majority of LOH cases, leading to the suggestion that NKX3.1 functions as a haploinsufficient tumor suppressor (4-6). Due to its highly restricted expression in prostate epithelial cells, NKX3.1 has been suggested as a diagnostic marker of prostate carcinoma (7), and may have additional utility as a biomarker of metastatic lesions originating in the prostate (8).

  1. Bhatia-Gaur, R. et al. (1999) Genes Dev 13, 966-77.
  2. He, W.W. et al. (1997) Genomics 43, 69-77.
  3. Bowen, C. et al. (2000) Cancer Res 60, 6111-5.
  4. Magee, J.A. et al. (2003) Cancer Cell 3, 273-83.
  5. Voeller, H.J. et al. (1997) Cancer Res 57, 4455-9.
  6. Bethel, C.R. et al. (2006) Cancer Res 66, 10683-90.
  7. Epstein, J.I. et al. (2014) Am J Surg Pathol 38, e6-e19.
  8. Conner, J.R. and Hornick, J.L. (2015) Adv Anat Pathol 22, 149-67.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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