Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246

i kappa b   IκB-α   IκBα   MAD3  

No. Size Price
9246L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
9246S 100 µl ( 10 western blots ) ¥3,960.00 现货查询 购买询价 防伪查询
9246 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Guinea Pig,

Specificity / Sensitivity

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb detects endogenous levels of IκBα only when phosphorylated at Ser32/36.

Phospho-IκBα (Ser32/36) (5A5) Mouse mAb抗体只能检测内源的在Ser32/36 位点磷酸化的IκBα蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.

单克隆是通过合成人源的对应的IκBα Ser32/36位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated orTNF-α-treated (#2169, 20 ng/ml) for 5 minutes, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) or IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (上图) 或者 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (下图)研究未经处理或经TNF-α处理 (#2169, 20 ng/ml,5 min) 的NIH/3T3 细胞的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (上图) 和 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (下图).研究经TPA (#9905, 80 nM for 24 h)分化和 1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。


The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。磷酸化IκBα 的Ser32 和 Ser36位点后,蛋白水解酶体介导的降解使得 NF-κB释放激活并入核(3-7)。IκBα的磷酸化导致了Rel依赖的转录能够被很多胞外信号激活,包括炎症因子,生长因子和趋化因子 。能够磷酸化IκB这些活性位点的激酶已经得到了鉴定(8)。

  1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
  2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
  3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
  4. Brown, K. et al. (1995) Science 267, 1485-8.
  5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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