Cell Signaling Technology

Product Pathways - MAPK Signaling

PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA Kit #91959

elisa   p90RSK   p90S6k   rsk   rsk1   screen  

REACTIVITY
H R Mk

No. Size Price
91959C 1 Kit ( 96 assays ) ¥6,345.00 现货查询 购买询价
Product Includes Volume Solution Color
ELISA Sample Diluent 25 ml Blue
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) 1 ea Red (Lyophilized)
Detection Antibody Diluent 11 ml Green
HRP Diluent 11 ml Red
RSK Mouse Detection mAb 1 ea Green (Lyophilized)
STOP Solution 11 ml Colorless
TMB Substrate #7004 11 ml Colorless
ELISA Wash Buffer (20X) 25 ml Colorless
Cell Lysis Buffer (10X) #9803 15 ml Yellowish
Sealing Tape 2 sheets
Phospho-p90RSK (Thr359) Rabbit mAb Coated Microwells 96 tests

Specificity / Sensitivity

PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA Kit detects endogenous levels of p90RSK protein phoshorylated at Thr359 in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of p90RSK protein phosphorylated at Thr359. A phospho-p90RSK (Thr359) rabbit antibody has been coated onto the microwells. After incubation with cell lysates, phospho-p90RSK proteins can be captured by the coated antibody. Following extensive washing, a p90RSK mouse antibody is added to detect the captured phospho-p90RSK protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of p90RSK phosphorylated at Thr359.

Antibodies in this kit are custom formulations specific to kit.

ELISA - Western correlation

ELISA - Western correlation

Figure 1: Phospho-p90RSK (Thr359) protein from untreated (-) and TPA treated (+) HeLa cells is detected by PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA kit. The absorbance readings at 450 nm are shown in the top figure,

while the corresponding Western blot using Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb #8753 is shown in the bottom figure.

Sensitivity

Sensitivity

Figure 2. The relationship between the protein concentration of lysates from untreated or TPA-treated HeLa cells, and the absorbance at 450 nm as detected by PathScan® Phospho-p90RSK (Thr359) Sandwich ELISA Kit is shown. Starved HeLa cells (85% confluent) were untreated or treated with TPA #4174 (200 nM, 15 minutes, 37ºC), and then lysed with cell lysis buffer.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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