Product Pathways - Tyrosine Kinase / Adaptors
EphA1 (D6V7I) Rabbit mAb #90673
|90673S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|90673||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
EphA1 (D6V7I) Rabbit mAb recognizes endogenous levels of total EphA1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to the extracellular domain of human EphA1 protein.
The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5).
The EphA1 receptor preferentially binds ephrin-A1 as a ligand (6). This ligand-receptor interaction stimulates EphA1 signaling and regulates cell morphology and motility through the ILK-RhoA-ROCK pathway (7). The EphA1 gene has been associated with late-onset Alzheimer's diseases (8,9). The role of EphA1 in cancer development falls into two opposite categories. In some type of cancer such as prostate, gastric and liver cancer, high expression of EphA1 associates with cancer metastasis and invasion (10-12). For other types of cancers, such as colon cancer and nonmelanoma skin cancer, down-regulation of the protein correlates with cancer development (13,14). The bidirectional signaling modulation of Ephrin-Ephrin receptor interaction might contribute this paradox phenomena (15).
- Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
- Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
- Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
- Holder, N. and Klein, R. (1999) Development 126, 2033-44.
- Brückner, K. et al. (1997) Science 275, 1640-3.
- Coulthard, M.G. et al. (2001) Growth Factors 18, 303-17.
- Yamazaki, T. et al. (2009) J Cell Sci 122, 243-55.
- Naj, A.C. et al. (2011) Nat Genet 43, 436-41.
- Hollingworth, P. et al. (2011) Nat Genet 43, 429-35.
- Wang, Y. et al. (2016) J Exp Clin Cancer Res 35, 65.
- Herath, N.I. et al. (2009) Br J Cancer 100, 1095-102.
- Hafner, C. et al. (2006) Mod Pathol 19, 1369-77.
- Pasquale, E.B. (2008) Cell 133, 38-52.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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