Cell Signaling Technology

Product Pathways - Adhesion

TACSTD2/TROP2 (D1W5W) Rabbit mAb #90540

No. Size Price
90540S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
90540 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45-65 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TACSTD2/TROP2 (D1W5W) Rabbit mAb recognizes endogenous levels of total TACSTD2/TROP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human TACSTD2/TROP2 protein.

IP

IP

Immunoprecipitation of TACSTD2 protein from UACC-812 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TACSTD2/TROP2 (D1W5W) Rabbit mAb. Western blot analysis was performed using TA CSTD2/TROP2 (D1W5W) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TACSTD2/TROP2 (D1W5W) Rabbit mAb.

Background

TROP2 is a transmembrane glycoprotein encoded by gene TACSTD2 (tumor associated calcium signal transducer 2). TROP2 was first discovered as a biomarker of invasive trophoblast cells and later reported in many types of cancer cells as well as in various organs during development, and adult stem cells during homeostasis (1, 2). TROP2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site and PKC phosphoryaltion site (Ser303) (1-4). TROP2 functions by regulating multiple signaling pathways including-interaction of extracellular domain with integrin beta1 to regulate FAK signaling, association of its transmembrane domain with claudin1 and claudin7 for tight junction formation, as well as regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway (1,2, 5-8). All of these functions are important for its role in tumor proliferation, metastasis and invasion (1,2). PKC can phosphorylate TROP2 at Ser303; the phosphorylation changes the cytoplasmic tail conformation and further promotes its signaling (9). TROP2 can be activated through intramembrane proteolysis first by TACE, followed further cleavage by presenilin 1 and presenilin2. The proteolysis process is required for its role in tumor cell proliferation (10,11).

  1. McDougall, A.R. et al. (2015) Dev Dyn 244, 99-109.
  2. Shvartsur, A. and Bonavida, B. (2015) Genes Cancer 6, 84-105.
  3. El Sewedy, T. et al. (1998) Int J Cancer 75, 324-30.
  4. Linnenbach, A.J. et al. (1993) Mol Cell Biol 13, 1507-15.
  5. Trerotola, M. et al. (2013) Cancer Res 73, 3155-67.
  6. Trerotola, M. et al. (2015) Oncotarget 6, 14318-28.
  7. Nakatsukasa, M. et al. (2010) Am J Pathol 177, 1344-55.
  8. Cubas, R. et al. (2010) Mol Cancer 9, 253.
  9. Pavšič, M. et al. (2015) Sci Rep 5, 10324.
  10. Stoyanova, T. et al. (2012) Genes Dev 26, 2271-85.
  11. Ju, X. et al. (2016) Cancer Res , .

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications