Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Epidermal Growth Factor (hEGF) #8916

EGF   EGF Receptor   EGFR   Epidermal Growth Factor   hEGF   Human EGF  

No. Size Price
8916SC 50 µg ( With Carrier ) ¥2,322.00 现货查询 购买询价 防伪查询
8916SF 50 µg ( Carrier Free ) ¥2,386.00 现货查询 购买询价 防伪查询
8916 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human EGF (hEGF) Asn971-Arg1023 (Accession #NM_0011963) was produced in E. coli at Cell Signaling Technology.

在Cell Signaling Technology公司的重组人源EGF (hEGF)天冬氨酸971-精氨酸1023(编号NM_0011963)经在大肠杆菌中表达生产。

Molecular Characterization

Recombinant hEGF has a Met on the amino terminus and has a calculated MW of 6353. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSDS of recombinant hEGF was verified by amino acid sequencing.

重组人源EGF在N-氨基末端有一个甲基化,分子量为6353. DTT还原和非还原蛋白游离基团为6kDa多肽。 预期在重组人源EGF的N-氨基末端MNSDS由氨基酸测序验证。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEGF. All lots are greater than 98% pure.

6 μg还原和非还原的重组人源EGF 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。


The bioactivity of recombinant hEGF was determined in a MCF10A cell proliferation assay. The ED50 of each lot is between 0.10-0.60 ng/ml.

重组人源EGF的生物活性由MCF10A细胞增殖实验测定。其每个批次的再ED50在 0.10-0.60 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hEGF and staining overnight with Coomassie Blue.重组hFGF basic 的纯度用SDS-PAGE验证,6 µg 还原(-)和未还原(-)重组hFGF basic 电泳后考马斯蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF for 10 minutes, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper) and EGF Receptor Antibody #2232 (lower).用Phospho-EGF Receptor (Tyr1173) (53A5)兔单抗#4407(上) 和EGF Receptor 抗体#2232(下) 对未处理或经过hEGF处理10分钟的A-431细胞提取物进行western blot实验。



The proliferation of MCF10A cells treated with increasing concentrations of hEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验浓度逐渐增长的hFGF basic 对MCF10A细胞增殖能力的影响。细胞在孵育24小时后,用BrdU处理4小时。BrdU掺入使用ELISA验证并检验OD450-OD690 。


Less than 0.01 ng endotoxin/1 μg hEGF.

每1 μg hEGF中所含内毒素小于0.01 ng。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每1 μg hEGF由含有20 μg BSA的pH 7.2的PBS 0.22微米过滤冻干。无载体:pH 7.2的PBS 0.22微米过滤冻干。


EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

EGF存在于上皮细胞,成纤维细胞和许多其他的细胞类型中(1,2)。低分子量可溶型EGF由一个分子量超过130,000的跨膜前体蛋白经蛋白水解生成(1,2)。可溶型和跨膜EGF都是有活性的(2)。EGF可以诱导多种细胞包括肿瘤来源的细胞增殖,分化和存活(1-3)。EGF家族和ErbB/HER EGF受体家族有许多成员。EGF结合ErbB1/HER1诱导同源二聚化或与其他ErbB/HER成员的异源二聚化(1)。EGF,EGF家族成员,EGF受体和它们的信号通路涉及到多种癌症,可以作为介入治疗的靶点(1,2)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :