Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ORF1p (D3W9O) Rabbit mAb #88701

L1   L1RE1   LINE-1  

No. Size Price
88701S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
88701 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 42 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

ORF1p (D3W9O) Rabbit mAb recognizes endogenous levels of total ORF1p protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human ORF1p protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ORF1p (D3W9O) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

Background

LINE-1, also known as L1, is a non-long terminal repeat (non-LTR) retrotransposon with hundreds of thousands of copies in the human genome (1, 2). Like all non-LTRs, L1 replicates by target-primed reverse transcription (TPRT) (3). The L1 retrotransposon encodes two proteins critical to this process: ORF1p and ORF2p. ORF2p contributes to endonuclease and reverse transcriptase activity, while ORF1p acts as a nucleic acid chaperone that binds RNA (4-8). Many types of cancers have been shown to have L1 insertions within tumor suppressor genes, disrupting their expression and contributing to tumorigenesis (9).

While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13).

  1. Kazazian, H.H. and Moran, J.V. (1998) Nat Genet 19, 19-24.
  2. Malik, H.S. et al. (1999) Mol Biol Evol 16, 793-805.
  3. Wilhelm, M. and Wilhelm, F.X. (2001) Cell Mol Life Sci 58, 1246-62.
  4. Scott, A.F. et al. (1987) Genomics 1, 113-25.
  5. Moran, J.V. et al. (1996) Cell 87, 917-27.
  6. Feng, Q. et al. (1996) Cell 87, 905-16.
  7. Martin, S.L. and Bushman, F.D. (2001) Mol Cell Biol 21, 467-75.
  8. Kolosha, V.O. and Martin, S.L. (1997) Proc Natl Acad Sci U S A 94, 10155-60.
  9. Lee, E. et al. (2012) Science 337, 967-71.
  10. Rodić, N. et al. (2015) Nat Med 21, 1060-4.
  11. Doucet-O'Hare, T.T. et al. (2015) Proc Natl Acad Sci U S A 112, E4894-900.
  12. Chen, L. et al. (2012) Breast Cancer Res Treat 136, 129-42.
  13. Rodić, N. et al. (2014) Am J Pathol 184, 1280-6.
  14. Cook, P.R. et al. (2015) Proc Natl Acad Sci U S A 112, 4298-303.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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