Cell Signaling Technology

Product Pathways - Metabolism

STEAP1 (D8B2V) Rabbit mAb #88677

No. Size Price
88677S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
88677 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 30 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

STEAP1 (D8B2V) Rabbit mAb recognizes endogenous levels of total STEAP1 protein. This antibody also recognizes overexpressed STEAP1B isoform 1 protein and may recognize endogenous STEAP1B isoform 1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human STEAP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from LnCAP cells and human prostate tissue using STEAP1 (D8B2V) Rabbit mAb.

IP

IP

Immunoprecipitation of STEAP1 from LnCap cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is STEAP1 (D8B2V) Rabbit mAb. Western blot analysis was performed using STEAP1 (D8B2V) Rabbit mAb.

Background

Six transmembrane epithelial antigen of the prostate 1 (STEAP1) is a transmembrane protein abundantly expressed in normal prostate epithelial cells (1). It is a metalloreductase that reduces Fe3+ to Fe2+ and Cu2+ to Cu1+ (2). STEAP1 is also reportedly overexpressed in multiple tumor types, and has been used as a cell surface biomarker for prostate cancer and Ewing's sarcoma (3-6). Peptides derived from STEAP1 protein have been shown to induce an antigen-specific cytotoxic T lymphocyte (CTL) response that can kill tumor cells expressing STEAP1 (7). These finding are the basis for research studies examining the efficacy of antibody-drug conjugates (ADCs) targeting STEAP1 (8,9).

  1. Hubert, R.S. et al. (1999) Proc Natl Acad Sci U S A 96, 14523-8.
  2. Ohgami, R.S. et al. (2006) Blood 108, 1388-94.
  3. Grunewald, T.G. et al. (2012) Mol Cancer Res 10, 52-65.
  4. Grunewald, T.G. et al. (2012) Ann Oncol 23, 2185-90.
  5. Ihlaseh-Catalano, S.M. et al. (2013) Histopathology 63, 678-85.
  6. Gomes, I.M. et al. (2014) Urol Oncol 32, 53.e23-9.
  7. Rodeberg, D.A. et al. (2005) Clin Cancer Res 11, 4545-52.
  8. Williams, S.P. et al. (2016) Oncotarget 7, 25103-12.
  9. Sukumaran, S. et al. (2016) AAPS J , .

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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