Cell Signaling Technology

Product Pathways - Apoptosis

MFF Antibody #86668

MFF   Mitochondrial Fission Factor  

No. Size Price
86668S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
86668 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 25, 27, 30, 35 Rabbit
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

MFF Antibody recognizes endogenous levels of total MFF protein. Based upon sequence alignment, this antibody is predicted to react with isoforms 1-5 of human MFF protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys52 of human MFF protein, isoform 1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MFF Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human MFF (+), using MFF Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

IP

IP

Immunoprecipitation of MFF from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MFF Antibody. Western blot analysis was performed using MFF Antibody.

Background

Mitochondrial fission factor (MFF) is a tail-anchored protein that resides within the outer mitochondrial membrane and is part of the mitochondrial fission complex. MFF participates in mitochondrial fission by serving as one of multiple receptors for the GTPase dynamin-related protein 1 (Drp1) (1-4). Research studies have also shown that MFF is a peroxisomal membrane protein and participates in peroxisome fission by serving as a receptor for another GTPase, dynamin-like protein 1 (5,6).

Research studies have demonstrated that the ability of MFF to drive acute mitochondrial fission in response to mitochondrial stress is controlled by AMPK-dependent phosphorylation. AMPK directly phosphorylates MFF at two sites to allow for enhanced recruitment of Drp1 to the mitochondra (7). Multiple isoforms of MFF are expressed as a result of alternative splicing (8). One of the major phosphoacceptor sites of MFF (Ser172 of human isoform 1/Ser146 of human isoforms 2-5) lies within an AMPK phsophorylation motif that spans the boundary of differentially spliced exons of MFF isoforms, suggesting that MFF splice isoforms are phosphorylated by AMPK to varying degrees.

  1. Liu, R. and Chan, D.C. (2015) Mol Biol Cell 26, 4466-77.
  2. Shen, Q. et al. (2014) Mol Biol Cell 25, 145-59.
  3. Losón, O.C. et al. (2013) Mol Biol Cell 24, 659-67.
  4. Otera, H. et al. (2010) J Cell Biol 191, 1141-58.
  5. Itoyama, A. et al. (2013) Biol Open 2, 998-1006.
  6. Gandre-Babbe, S. and van der Bliek, A.M. (2008) Mol Biol Cell 19, 2402-12.
  7. Toyama, E.Q. et al. (2016) Science 351, 275-81.
  8. Ducommun, S. et al. (2015) Cell Signal 27, 978-88.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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