Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

PD-1 (D4W2J) XP® Rabbit mAb #86163

autostainer   Bond   CD279   checkpoint blockade   Leica   PD-1   PD1   PDCD1   programmed cell death protein 1   Rx   T cell co-inhibitory receptors  

No. Size Price
86163S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
86163T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
86163 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 52-65 Rabbit
IP 1:200
IHC-P 1:200
F 1:200
IHC-Bond 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IHC-Bond=IHC-Leica® Bond™,

Specificity / Sensitivity

PD-1 (D4W2J) XP® Rabbit mAb recognizes endogenous levels of total PD-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (D4W2J) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from human CD4+ T cells, MOLT-4, and Jurkat cells using PD-1 (D4W2J) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from human blood and stimulated for 9 days using beads coated with CD3 and CD28 antibodies in the presence of human interleukin-2 (hIL-2) #8907 (6.7 ng/ml).

IP

IP

Immunoprecipitation of PD-1 protein from Molt-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PD-1 (D4W2J) XP® Rabbit mAb. Western blot analysis was performed using PD-1

(D4W2J) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-1 (D4W2J) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using PD-1 (D4W2J) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells, CD3/CD28-treated (72 hr; left column) or untreated (right column), using PD-1 (D4W2J) XP® Rabbit mAb #86163 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (bottom row), and co-stained with a CD3 antibody.  Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IHC-Leica Bond

IHC-Leica Bond

Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using PD-1 (D4W2J) XP® Rabbit mAb performed on the Leica® Bond™ Rx.

Background

The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).

  1. Ishida, Y. et al. (1992) EMBO J 11, 3887-95.
  2. Shinohara, T. et al. (1994) Genomics 23, 704-6.
  3. Nishimura, H. et al. (1999) Immunity 11, 141-51.
  4. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  5. Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.
  6. Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.
  7. Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.
  8. Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.
  9. Dong, H. et al. (2002) Nat Med 8, 793-800.
  10. Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

LEICA is a registered trade​mark of Leica Microsystems IR GmbH.

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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