Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb #8566

GSK-3 alpha   GSK-3 beta   GSK-3alpha   GSK-3beta  

No. Size Price
8566S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
8566 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 46 GSK-3beta, 51 GSK-3alpha Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb recognizes endogenous levels of GSK-3α and GSK-3β proteins only when phosphorylated at Ser21 or Ser9, respectively.

Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb兔单抗可以分别识别Ser21或Ser9位点磷酸化的内源性GSK-3α and GSK-3β蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3α protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from GSK-3α (-/-) and GSK-3β (-/-) mouse embryonic fibroblasts (MEF), untreated or insulin-treated (100 ng/ml, 20 min) , using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Western blot 分析GSK-3α (-/-) 或 GSK-3β (-/-) (泳道 5,6) 小鼠胚胎成纤维细胞 (MEFs), 未处理组或 胰岛素处理组(100 ng/ml, 20 min), 所用抗体为Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb兔单抗 (上) 和GSK-3α/β (D75D3) XP ® Rabbit mAb 兔单抗#5676 (下). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) 细胞是加拿大多伦多大学的Dr. Jim Woodgett友情赠送).

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, λ phosphatase- or PDGF-treated (100 μg/ml, 15 min), and PC-3 cells, untreated or λ phosphatase-treated, using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower).

Western blot 分析COS-7 细胞提取物, λ 磷酸酶或PDGF处理组(100 μg/ml, 15分钟), 和 PC-3 细胞,未处理组或λ磷酸酶处理组, 所用抗体为 Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb 兔单抗(上)和GSK-3α/β (D75D3) XP ® Rabbit mAb 兔单抗#5676 (下)。


Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

Application References

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