Cell Signaling Technology

Product Pathways - Adhesion

PathScan® Total EpCAM Sandwich ELISA Kit #85626

elisa   EPCAM   screen  

REACTIVITY
H

No. Size Price
85626C 1 Kit ( 96 assays ) ¥6,346.00 现货查询 购买询价 防伪查询
Product Includes Volume Solution Color
ELISA Sample Diluent 25 ml Blue
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) 1 ea Red (Lyophilized)
Detection Antibody Diluent 11 ml Green
HRP Diluent 11 ml Red
EpCAM Mouse Detection mAb 1 ea Green (Lyophilized)
EpCAM Rabbit mAb Coated Microwells 96 tests
STOP Solution #7002 11 ml Colorless
TMB Substrate #7004 11 ml Colorless
ELISA Wash Buffer (20X) 25 ml Colorless
Cell Lysis Buffer (10X) #9803 15 ml Yellowish
Sealing Tape 2 sheets

Specificity / Sensitivity

PathScan® Total EpCAM Sandwich ELISA Kit detects endogenous levels of EpCAM protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total EpCAM Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of EpCAM protein. An EpCAM rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the EpCAM proteins are captured by the coated antibody. Following extensive washing, an EpCAM mouse detection mAb is added to detect captured EpCAM proteins. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of EpCAM protein.

Antibodies in the kit are custom formulations specific to the kit.

ELISA - Western correlation

ELISA - Western correlation

Figure 1. Detection of EpCAM protein MCF7, HT-29, HeLa cells is examined by using the PathScan® Total EpCAM Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using EpCAM (D1B3) Rabbit mAb #2626 is shown in the bottom figure.

Sensitivity

Sensitivity

Figure 2. The relationship between protein concentration of lysates from HT-29 cells and the absorbance at 450 nm as detected by the PathScan® Total EpCAM Sandwich ELISA Kit is shown. Unstarved HT-29 cells (85% confluence) were harvested and then lysed.

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).

  1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
  2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
  3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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