Product Pathways - Adhesion
PathScan® Total EpCAM Sandwich ELISA Kit #85626
|Product Includes||Volume||Solution Color|
|ELISA Sample Diluent||25 ml||Blue|
|Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated)||1 ea||Red (Lyophilized)|
|Detection Antibody Diluent||11 ml||Green|
|HRP Diluent||11 ml||Red|
|EpCAM Mouse Detection mAb||1 ea||Green (Lyophilized)|
|EpCAM Rabbit mAb Coated Microwells||96 tests|
|STOP Solution||11 ml||Colorless|
|TMB Substrate #7004||11 ml||Colorless|
|ELISA Wash Buffer (20X)||25 ml||Colorless|
|Cell Lysis Buffer (10X) #9803||15 ml||Yellowish|
|Sealing Tape||2 sheets|
Specificity / Sensitivity
PathScan® Total EpCAM Sandwich ELISA Kit detects endogenous levels of EpCAM protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The PathScan® Total EpCAM Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of EpCAM protein. An EpCAM rabbit mAb has been coated onto the microwells. After incubation with cell lysates, the EpCAM proteins are captured by the coated antibody. Following extensive washing, an EpCAM mouse detection mAb is added to detect captured EpCAM proteins. Anti-mouse, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of EpCAM protein.
Antibodies in the kit are custom formulations specific to the kit.
ELISA - Western correlation
Figure 1. Detection of EpCAM protein MCF7, HT-29, HeLa cells is examined by using the PathScan® Total EpCAM Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blot using EpCAM (D1B3) Rabbit mAb #2626 is shown in the bottom figure.
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
- Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
- Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
- Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.
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- 2626 EpCAM (D1B3) Rabbit mAb
- 2929 EpCAM (VU1D9) Mouse mAb
- 3599 EpCAM Antibody
- 36746 EpCAM (D4K8R) XP® Rabbit mAb
- 7004 TMB Substrate
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 9803 Cell Lysis Buffer (10X)
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
- 9998 BSA
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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