Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Palladin (D9H2) Rabbit mAb #8518

No. Size Price
8518S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8518 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 140, 90 Rabbit IgG
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Palladin (D9H2) Rabbit mAb recognizes endogenous levels of total palladin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1043 of human palladin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SNB19, HeLa and ACHN cells using Palladin (D9H2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Palladin (D9H2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Palladin (D9H2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Palladin (D9H2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Palladin (D9H2) Rabbit mAb.

Background

The actin-associated scaffold protein, palladin, is associated with multiple actin-rich structures, and plays a role in cellular migration and invasion (1). Palladin regulates the formation of invasive structures called podosomes and invadopodia (1-3). Alternative splicing results in at least three palladin isoforms, including the widely expressed 140 kDa and 90 kDa isoforms (2). Palladin has been shown to promote invasion in basal-like breast carcinoma models (4). Palladin is also upregulated in injured kidneys, and promotes migration of kidney cells to facilitate repair (5). In differentiating muscle cells, palladin regulates migration and myogenesis (6). Several research studies have focused on palladin expression and function in cancer-associated fibroblasts (CAFs). Researchers have shown that expression of palladin in stromal fibroblasts of pancreatic ductal adenocarcinoma is an indication of the effectiveness of chemotherapy (7). The cancer associated transcription factor Twist1 may require palladin and collagen alpha1 for its metastatic effect in fibroblasts (8).

  1. Goicoechea, S.M. et al. (2008) Eur J Cell Biol 87, 517-25.
  2. Najm, P. and El-Sibai, M. (2014) Cell Adh Migr 8, 29-35.
  3. Goicoechea, S.M. et al. (2014) Oncogene 33, 1265-73.
  4. von Nandelstadh, P. et al. (2014) Mol Biol Cell 25, 2556-70.
  5. Chang, E.H. et al. (2015) Sci Rep 5, 7695.
  6. Nguyen, N.U. and Wang, H.V. (2015) PLoS One 10, e0124762.
  7. Sato, D. et al. (2016) PLoS One 11, e0152523.
  8. García-Palmero, I. et al. (2016) Oncogene , .

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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