Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb #84192

No. Size Price
84192S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
84192 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 82 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-FoxO1 (Ser256) (E1F7T) recognizes endogenous levels of FoxO1 protein only when phosphorylated at Ser256. The antibody cross-reacts with overexpressed FoxO4 phosphorylated at Ser193 and may cross-react with overexpressed FoxO3a phosphorylated at Ser253. The antibody also cross-reacts with a protein of unknown origin around 160kD.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser256 of human FoxO1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated or phosphatase-treated, using Phospho-Fox01 (Ser256) (E1F7T) Rabbit mAb (upper), FoxO1 (C29H4) Rabbit mAb #2880 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, insulin-treated (100 nM, 15 min) or LY294002-treated #9901 (50 μM, 2 hr), using Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb (upper) or FoxO1 (C29H4) Rabbit mAb #2880 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells expressing either non-targeting shRNA (shNT) or Fox01 shRNA (shFoxO1) using Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb (upper) and FoxO3a (75D8) Rabbit mAb #2497 (lower). 293T shNT and shFoxO1 cells were kindly provided by Dr. Anne Brunet, Stanford University, Stanford CA.

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

  1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
  2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
  3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
  4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
  5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
  6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
  7. Seoane, J. et al. (2004) Cell 117, 211-23.
  8. Arden, K.C. (2004) Mol Cell 14, 416-8.
  9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.

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