Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) #83882

No. Size Price
83882S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
83882T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
83882 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 45-80 Rabbit IgG
IP 1:100
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total TIM-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro137 of mouse TIM-3 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse thymus using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of TIM-3 from Raw264.7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) XP® Isotype Control #3900, and lane 3 is TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific). Western blot analysis was performed using

TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).

Background

T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).

  1. Monney, L. et al. (2002) Nature 415, 536-41.
  2. Sánchez-Fueyo, A. et al. (2003) Nat Immunol 4, 1093-101.
  3. Sabatos, C.A. et al. (2003) Nat Immunol 4, 1102-10.
  4. Jones, R.B. et al. (2008) J Exp Med 205, 2763-79.
  5. Sakuishi, K. et al. (2010) J Exp Med 207, 2187-94.
  6. Chiba, S. et al. (2012) Nat Immunol 13, 832-42.
  7. Huang, Y.H. et al. (2015) Nature 517, 386-90.
  8. Zhu, C. et al. (2005) Nat Immunol 6, 1245-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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