Product Pathways - Lymphocyte Signaling
TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) #83882
|83882S||100 µl ( 10 western blots )||￥3,580.00 现货查询||购买询价|
|83882T||20 µl ( 2 western blots )||￥1,400.00 现货查询||购买询价|
|83882||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),
Specificity / Sensitivity
TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total TIM-3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro137 of mouse TIM-3 protein.
Immunohistochemical analysis of paraffin-embedded mouse spleen using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).
Immunohistochemical analysis of paraffin-embedded mouse thymus using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).
Western blot analysis of extracts from various cell lines using TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of TIM-3 from Raw264.7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) XP® Isotype Control #3900, and lane 3 is TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific). Western blot analysis was performed using
TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific).
T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).
- Monney, L. et al. (2002) Nature 415, 536-41.
- Sánchez-Fueyo, A. et al. (2003) Nat Immunol 4, 1093-101.
- Sabatos, C.A. et al. (2003) Nat Immunol 4, 1102-10.
- Jones, R.B. et al. (2008) J Exp Med 205, 2763-79.
- Sakuishi, K. et al. (2010) J Exp Med 207, 2187-94.
- Chiba, S. et al. (2012) Nat Immunol 13, 832-42.
- Huang, Y.H. et al. (2015) Nature 517, 386-90.
- Zhu, C. et al. (2005) Nat Immunol 6, 1245-52.
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- 14166 Hematoxylin
- 14177 SignalStain® Mounting Medium
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- 15019 Animal-Free Blocking Solution (5X)
- 5425 Normal Goat Serum
- 7003 20X LumiGLO® Reagent and 20X Peroxide
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 8059 SignalStain® DAB Substrate Kit
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
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XP is a registered trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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