Cell Signaling Technology

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Claudin-3 (D7A3O) Rabbit mAb #83609

No. Size Price
83609S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
83609 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 20 Rabbit IgG
IP 1:200
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Claudin-3 (D7A3O) Rabbit mAb recognizes endogenous levels of total claudin-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro206 of human claudin-3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Claudin-3 (D7A3O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of T-47D (left) and Saos-2 (right) cells using Claudin-3 (D7A3O) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IP

IP

Immunoprecipitation of claudin-3 protein from T-47D cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Claudin-3 (D7A3O) Rabbit mAb. Western blot analysis was performed using Claudin-3 (D7A3O) Rabbit mAb.

Background

Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6).

Claudin-3 is the product of an intronless gene (CLDN3) whose expression is markedly dysregulated in distinct cancer subtypes, including ovarian and breast carcinomas (7-9). Somewhat paradoxically, reduced claudin-3 expression is also a hallmark of a subset of breast tumors known as the claudin-low subtype, in which reduced claudin-3 expression was shown to correlate with a phenotype characteristic of cells undergoing epithelial-mesenchymal transition (10).

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.
  3. Hewitt, K.J. et al. (2006) BMC Cancer 6, 186.
  4. Brandner, J.M. et al. (2002) Eur J Cell Biol 81, 253-63.
  5. Krämer, F. et al. (2000) Hum Genet 107, 249-56.
  6. Swisshelm, K. et al. (1999) Gene 226, 285-95.
  7. Choi, Y.L. et al. (2007) Histol Histopathol 22, 1185-95.
  8. Hudson, L.G. et al. (2008) Clin Exp Metastasis 25, 643-55.
  9. Todd, M.C. et al. (2015) Oncol Lett 10, 156-162.
  10. Herschkowitz, J.I. et al. (2012) Proc Natl Acad Sci U S A 109, 2778-83.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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