Cell Signaling Technology

Product Pathways - Protein Stability

UBE2O Antibody #83393

E2   E2-230K   E2-E3   UBE2O   Ubiquitin conjugating enzymes  

No. Size Price
83393S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
83393 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 200 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine,

Specificity / Sensitivity

UBE2O Antibody recognizes endogenous levels of total UBE2O protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val576 of human UBE2O protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBE2O Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2O protein (hUBE2O-Myc/DDK; +), using UBE2O Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower).

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

UBE2O (E2-230K) is a unique member of the E2 family of ubiquitin-conjugating enzymes in that it functions as an E2-E3 hybrid enzyme (2). Research studies have demonstrated that UBE2O expression is regulated during erythroid differentiation, which suggests that its enzymatic activity participates in shaping the architecture of the erythrocyte proteome (3). UBE2O has also been implicated in BMP-7-induced adipocyte differentiation through its regulation of SMAD6 monoubiquitination (4).

  1. Hershko, A. (1988) J Biol Chem 263, 15237-40.
  2. Berleth, E.S. and Pickart, C.M. (1996) Biochemistry 35, 1664-71.
  3. Wefes, I. et al. (1995) Proc Natl Acad Sci U S A 92, 4982-6.
  4. Zhang, X. et al. (2013) EMBO J 32, 996-1007.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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