渲染靶标:SSR
Render Timestamp: 2025-01-21T18:42:24.612Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-08-01 15:24:47.642
Product last modified at: 2025-01-01T09:01:48.791Z
1% for the Planet 标识
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Homer1 Antibody #8231

Filter:
  • WB
Western Blotting Image 1: Homer1 Antibody
Western blot analysis of extracts from mouse and rat brain tissue using Homer1 Antibody.

To Purchase # 8231S

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 46
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Homer1 Antibody detects endogenous levels of total Homer1 protein. This antibody may also detect isoform Homer1c.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu130 of human Homer1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Homer1, Homer2 and Homer3 are scaffolding proteins, composed of an EVH protein–binding domain, a coiled-coil domain and a leucine zipper domain. The EVH domain is a protein-protein binding module that binds to the proline-rich motifs PPXXF, PPXF, and LPSSP of G protein–coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels (1-2). The coiled-coil and the leucine zipper domains cause multimerization of Homers and assemble signaling proteins complexes. The Homer1 gene encodes a short isoform (Homer1a, aa 1-186) and two long isoforms (Homer1b, aa 1-354; Homer1c, aa 1-366). Homer1a lacks the coiled-coil domain and leucine zipper, antagonizing multimerization of Homers and thus disassembling signaling proteins complexes (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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