Cell Signaling Technology

Product Pathways - Development

CUTL1 Antibody #81557

sc-13024   sc-6327  

No. Size Price
81557S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
81557 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 200 Rabbit
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CUTL1 Antibody recognizes endogenous levels of total CUTL1 protein. It recognizes the p200 isoform (the full-length) and is not expected to recognize the p110 and p75 isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln639 of human CUTL1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HCT-15, and K-562 cells using CUTL1 Antibody.

Background

CUTL1 (Cut-like 1), also known as CUX1 (Cut homeobox 1) (CUX1), is a transcription factor that has been implicated in many cellular processes in different tissues, such as cell migration, neuronal differentiation, and DNA repair (1-5). CUTL1 expression and activities are altered in cancer. Research studies have shown the CUTL1 gene to be a frequent target of loss-of-heterozygocity in various cancers (6,7). On the other hand, CUTL1 expression is elevated in many cancers and is associated with shorter disease-free survival (8). These accumulating evidence suggest that decreased CUTL1 expression promote tumor initiation and increased CUTL1 expression facilitates tumor progression (9). While full-length CUTL1 is about 200 kDa (p200), short forms p110 and p75 can also be generated by proteolytic processing and alternative transcription initiation site, respectively (10, 11).

  1. Rodríguez-Tornos, F.M. et al. (2016) Neuron 89, 494-506.
  2. Ramdzan, Z.M. et al. (2015) Oncotarget 6, 3613-26.
  3. Ramdzan, Z.M. et al. (2014) PLoS Biol 12, e1001807.
  4. Kedinger, V. et al. (2009) J Biol Chem 284, 27701-11.
  5. Vadnais, C. et al. (2012) Nucleic Acids Res 40, 4483-95.
  6. McNerney, M.E. et al. (2013) Blood 121, 975-83.
  7. Ramdzan, Z.M. and Nepveu, A. (2014) Nat Rev Cancer 14, 673-82.
  8. Michl, P. et al. (2005) Cancer Cell 7, 521-32.
  9. Hulea, L. and Nepveu, A. (2012) Gene 497, 18-26.
  10. Moon, N.S. et al. (2001) Mol Cell Biol 21, 6332-45.
  11. Goulet, B. et al. (2002) Cancer Res 62, 6625-33.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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