Cell Signaling Technology

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SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125

Boost   Detection   IHC   Immunohistochemistry   secondary   SignalStain  

No. Size Price
8125P 1 ml ( 10 slides ) ¥504.00 现货查询 购买询价 防伪查询
8125S 15 ml ( 150 slides ) ¥4,654.00 现货查询 购买询价 防伪查询
8125 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P Goat

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

SignalStain® Boost IHC Detection Reagent (HRP, Mouse) can be used to detect mouse primary antibodies in immunohistochemical assays. Some antibodies may require titration when used with this highly sensitive reagent.

该试剂用于在免疫沉淀中检测鼠源一抗。当用这种高灵敏度试剂时,一些抗体需要稀释。

Description

SignalStain® Boost IHC Detection Reagent (HRP, Mouse) is a ready-to-use solution intended for use in immunohistochemical assays to detect mouse primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Mouse) is a highly sensitive, one-step, polymer-based detection reagent that is specific for mouse IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.

是一种在免疫沉淀中用于检测鼠源一抗的现成溶液。 SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) 具有高灵敏度,仅一步,含有聚合物的检测试剂,特异性针对鼠IgG。在石蜡包埋和冻存的组织中,此溶液可以使目的物可见,并且它还可以与所有过氧化物底物同时使用

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb #4893 with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Mouse) (right).免疫组化分析石蜡包埋的人精原细胞瘤 ,所用抗体为g Nanog (1E6C4) Mouse mAb #4893 with biotin-based detection (左图)或SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) (右图).

Directions for Use

Equilibrate SignalStain® Boost IHC Detection Reagent (HRP, Mouse) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.

在使用前,将其平衡至室温。根据需要,在组织切片上滴1-3滴(40-120ul),在湿润的小室中室温孵育30分钟。洗涤切片,再使用过氧化物酶底物。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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