Cat. # | Size | Price | Inventory |
---|---|---|---|
8097S | 100 µl |
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 130 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Human, Mouse, Rat
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His900 of human TECPR1 protein.
Tectonin β-propeller repeat protein (TECPR1) was first identified as DKFZP434B0335, a human ortholog of the yeast protein Pex23p (1). TECPR1 contains a pleckstrin homology (PH) domain, β-propeller domain, and dysferlin domains. Research studies have shown that elevated expression of TECPR1 may be a potential marker for prostate cancer (2). In several independent studies, TECPR1 was shown to play a role in autophagy through interaction with Atg5 (3-5). Atg5 is a protein that is conjugated to the ubiquitin-like protein Atg12 and plays an essential role in autophagy (6). Initial studies suggested that TECPR1 plays a role in selective autophagy processes by targeting bacterial pathogens, as well as damaged mitochondria and protein aggregates (4). TECPR1 appears to be important for autophagosome maturation and promotes autophagosome fusion with the lysosome (5). Deletion of TECPR1 leads to an accumulation of autophagosomes (5).
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