Product Pathways - Jak/Stat Pathway
Stat1 (D1K9Y) Rabbit mAb (PE Conjugate) #80916
|80916S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|80916||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
Stat1 (D1K9Y) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Stat1 protein. This antibody also cross-reacts with an unidentified protein of 150 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro688 of human Stat1 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Stat1 (D1K9Y) Rabbit mAb #14994.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
- Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
- Durbin, J.E. et al. (1996) Cell 84, 443-50.
- Meraz, M.A. et al. (1996) Cell 84, 431-42.
- Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
- Frank, D.A. (1999) Mol Med 5, 432-56.
- Wen, Z. et al. (1995) Cell 82, 241-50.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 14994 Stat1 (D1K9Y) Rabbit mAb
- 25809 Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (PE Conjugate)
- 8062 Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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