Cell Signaling Technology

Product Pathways - Metabolism

IRG1 (D6H2Y) Rabbit mAb #77510

No. Size Price
77510S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
77510 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 53 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

IRG1 (D6H2Y) Rabbit mAb recognizes endogenous levels of total IRG1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly460 of human IRG1 protein.

Western Blotting

Western Blotting

Western blot analysis of lysates from THP-1 untreated (-) or treated with TPA (#4174, 50ng/ml) for 24 hours then LPS (5ug/ml) for 6 hours in fresh medium (+), using IRG1 (D6H2Y) Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118 (lower).

Background

IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6).

  1. Lee, C.G. et al. (1995) Immunogenetics 41, 263-70.
  2. Chen, B. et al. (2003) Mol Endocrinol 17, 2340-54.
  3. Cheon, Y.P. et al. (2003) Endocrinology 144, 5623-30.
  4. Michelucci, A. et al. (2013) Proc Natl Acad Sci U S A 110, 7820-5.
  5. Lampropoulou, V. et al. (2016) Cell Metab 24, 158-66.
  6. Cordes, T. et al. (2016) J Biol Chem 291, 14274-84.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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