Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb (PE Conjugate) #77366

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Rat, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Stat2 (D3P2P) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat2 protein only when phosphorylated at Tyr690.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr690 of human Stat2 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat2 (D3P2P) Rabbit mAb #88410.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of U266 cells, untreated (blue) or treated with IFN-α (green) using Phospho-Stat2 (Tyr690) (D3P2P) Rabbit mAb (PE Conjugate).

Background

Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5).

  1. Fu, X.Y. et al. (1992) Proc Natl Acad Sci U S A 89, 7840-3.
  2. Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7.
  3. Park, C. et al. (2000) Immunity 13, 795-804.
  4. Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80.
  5. Horvath, C.M. et al. (1996) Mol Cell Biol 16, 6957-64.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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