Product Pathways - NF-kB Signaling
IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred) #76041
|76041S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|76041||carrier free & custom formulation / quantity||email request|
|W||1:1000||Mouse,||Endogenous||75, 85||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,
Specificity / Sensitivity
IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred) recognizes endogenous levels of total mouse IκB-ζ protein. This antibody has weak reactivity for rat and human. Product #93726 is preferred for western blot.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly108 of mouse IκB-ζ protein.
Western blot analysis of extracts from J774A.1 and Raw 264.7 cell lines, untreated (-) or treated with LPS (1 μg/ml, 4 hr; +) using IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse IκB-ζ (mIκB-ζ; +) using IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred).
Immunoprecipitation of IκB-ζ from Raw 264.7 cells treated with LPS (1 μg/ml; 4 hr). Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred). Western blot was performed using IκB-ζ (D4I7C) Rabbit mAb (ChIP Preferred). Blots were developed using a confirmation specific secondary antibody to avoid reactivity with IgG.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 J774A.1 cells treated with LPS #14011 (1μg/mL, 4hours) and either 10 µl of IκB-ζ (D4I7C) Rabbit mAb (Mouse Specific) or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse CXCL2 Promoter Primers #39609, mouse LCN2 promoter primers, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
IκB-ζ (MAIL, INAP) is a unique IκB family member homologous to Bcl-3 and induced by IL-1 and Toll-like receptor (TLR) ligands (9-11). Like other family members, it contains carboxyl terminal ankyrin-repeats responsible for interaction with NF-κB, particularly p50. Unlike classical IκB family members (α, β, ε) which inhibit NF-κB translocation and are rapidly degraded upon cytokine treatment, IκB-ζ is cytokine-inducible and localized to the nucleus where it regulates NF-κB DNA binding and transactivation (12-14). Induction of IκB-ζ is required for TLR/IL-1 induction of a subset of NF-κB target genes, including IL-6 (15). However, the IκB-ζ can also inhibit transactivation of other targets, such as TNF-α (14,15).
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Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 39609 SimpleChIP® Mouse CXCL2 Promoter Primers
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7017 6-Tube Magnetic Separation Rack
- 7071 Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 8985 SimpleChIP® Mouse MYT-1 Promoter Primers
- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9004 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
- 9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9006 ChIP-Grade Protein G Magnetic Beads
- 9007 ChIP-Grade Protein G Agarose Beads
- 93726 IκB-ζ Antibody (Mouse Specific)
For Research Use Only. Not For Use In Diagnostic Procedures.
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