Cell Signaling Technology

Product Pathways - Transcription Factors

BCL11A (D4E3P) Rabbit mAb #75432

No. Size Price
75432S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
75432 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 100-145 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

BCL11A (D4E3P) Rabbit mAb recognizes endogenous levels of total BCL11A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val247 of human BCL11A protein.

IP

IP

Immunoprecipiation of BCL11A from RPMI 8226 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BCL11A (D4E3P) Rabbit mAb. Western blot analysis was performed using BCL11A (D4E3P) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BCL11A (D4E3P) Rabbit mAb.

Background

BCL11A is a zinc finger-containing transcriptional repressor that is important for normal hematopoiesis (1). Alternative splicing of the BCL11A transcript results in several isoforms of the protein (2). BCL11A is required for the early stages of B lineage commitment and mice lacking BCL11A fail to develop B cells (1). Mice deficient in BCL11A also fail to develop plasmacytoid dendritic cells (3). In addition, BCL11A regulates the switch from fetal to adult hemoglobin by repressing expression of fetal hemoglobin in adult erythroid cells (4). Since expression of fetal hemoglobin can decrease the severity of hemoglobin disorders in adults, BCL11A is a potential therapeutic target for these diseases (4). BCL11A was also recently identified as a component of the mammalian SWI/SNF complex (5). BCL11A is required for morphogenesis and terminal differentiation of dorsal spinal neurons (6).

  1. Liu, P. et al. (2003) Nat Immunol 4, 525-32.
  2. Liu, H. et al. (2006) Mol Cancer 5, 18.
  3. Ippolito, G.C. et al. (2014) Proc Natl Acad Sci U S A 111, E998-1006.
  4. Sankaran, V.G. et al. (2008) Science 322, 1839-42.
  5. Kadoch, C. et al. (2013) Nat Genet 45, 592-601.
  6. John, A. et al. (2012) Development 139, 1831-41.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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