Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

CaSR (D6D9V) Rabbit mAb #73303

No. Size Price
73303S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
73303 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 130, 260 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CaSR (D6D9V) Rabbit mAb recognizes endogenous levels of total CaSR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro368 of human CaSR protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from human kidney, human liver, and TT cells, using CaSR (D6D9V) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

IP

IP

Immunoprecipitation of CaSR from TT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CaSR (D6D9V) Rabbit mAb. Western blot analysis was performed using CaSR (D6D9V) Rabbit mAb.

Background

CaSR, the extracellular Calcium-Sensing Receptor, is a widely expressed G-protein coupled receptor (GPCR) involved in calcium homeostasis. CaSR operates as a sensor in parathyroid and kidney, and alterations in its activity have been shown to cause thyroid disease in humans (1). Activation of the receptor in response to extracellular calcium or other ligands causes activation of phospholipase C (PLC), release of IP3 and release of calcium from intracellular stores (2). Proinflammatory cytokines IL-1β and TNF-α increase CaSR gene expression in human thyroid and kidney cells through activation of the NF-κB pathway, and this pathway may be involved in hypocalcemia often seen in critically ill patients (3). Elevated calcium concentration and CaSR expression have been linked to proliferation and metastasis of skeletal metastatic prostate cancer cell lines (4). In intestinal epithelial cells, CaSR is involved in regulation of cyclic nucleotide metabolism and the fluid secretion that results in life-threatening fluid loss in response to intestinal pathogens (5). The interaction of CaSR with the actin-binding protein filamin may provide scaffolding for the organization of signaling pathways converging on the cytoskeleton, including CaSR-mediated MAPK pathway activation (6).

  1. Hofer, A.M. and Brown, E.M. (2003) Nat Rev Mol Cell Biol 4, 530-8.
  2. Cheng, S.X. et al. (2002) Am J Physiol Gastrointest Liver Physiol 283, G240-50.
  3. Canaff, L. and Hendy, G.N. (2005) J Biol Chem 280, 14177-88.
  4. Liao, J. et al. (2006) Cancer Res 66, 9065-73.
  5. Geibel, J. et al. (2006) Proc Natl Acad Sci U S A 103, 9390-7.
  6. Awata, H. et al. (2001) J Biol Chem 276, 34871-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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