Cell Signaling Technology

Product Pathways - Transcription Factors

Sox9 (D8G8H) Rabbit mAb (Alexa Fluor® 647 Conjugate) #71273

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Sox9 (D8G9H) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total Sox9 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Sox9 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Sox9 (D8G9H) Rabbit mAb #82630.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and A-204 cells (green) using Sox9 (D8G8H) Rabbit mAb (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-204 (left) or HeLa (right) cells using Sox9 (D8G8H) (Alexa Fluor® 647 Conjugate) Rabbit mAb (red). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (green pseudocolor).

Background

Sox9 is a transcription factor with an HMG-box DNA binding domain that has homology to the HMG domain of the mammalian testis-determining factor, SRY (1). Sox9 regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination (4). In addition, Sox9 is reportedly involved in the maintenance of adult stem cell populations, including multipotent neural stem cells (5), hair follicle stem cells (6), and mammary stem cells (7). Recent interest has focused on the role of Sox9 in tumor biology. For example, research studies have shown that Sox9 expression in lung adenocarcinoma induces a mesenchymal phenotype in tumor cells (8). Other research studies have shown that YAP1 induced upregulation of Sox9 confers cancer stem cell like properties on esophageal cancer cells (9). Moreover, Sox9 expression has been linked with several other tumor types including ovarian, prostate, and pancreatic malignancies (10-12).

  1. Mertin, S. et al. (1999) Nucleic Acids Res 27, 1359-64.
  2. Akiyama, H. et al. (2002) Genes Dev 16, 2813-28.
  3. Raspopovic, J. et al. (2014) Science 345, 566-70.
  4. De Santa Barbara, P. et al. (1998) Mol Cell Biol 18, 6653-65.
  5. Scott, C.E. et al. (2010) Nat Neurosci 13, 1181-9.
  6. Kadaja, M. et al. (2014) Genes Dev 28, 328-41.
  7. Guo, W. et al. (2012) Cell 148, 1015-28.
  8. Capaccione, K.M. et al. (2014) Oncotarget 5, 3636-50.
  9. Song, S. et al. (2014) Cancer Res 74, 4170-82.
  10. Raspaglio, G. et al. (2014) Gene 542, 173-81.
  11. Qin, G.Q. et al. (2014) Onco Targets Ther 7, 137-46.
  12. Shroff, S. et al. (2014) Hum Pathol 45, 456-63.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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