Cell Signaling Technology

Product Pathways - Companion Products

Anti-rabbit IgG, HRP-linked Antibody #7074

anti rabbit   rabbit hrp   sc-2004   sc-2030   sc-2054   sc-2313   secondary antibody  

Applications Species-Reactivity Sensitivity MW (kDa) Isotype
W Endogenous Goat

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Affinity purified goat anti-rabbit IgG (H&L) antibody is conjugated to horseradish peroxidase.

Affinity purified goat anti-rabbit IgG (H&L) antibody 偶联了HRP。

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.一抗与靶蛋白结合后,与HRP标记的二抗结合形成复合体。加入LumiGLO ®后,被酶催化分解后发出光信号。


This product has been optimized for use as a secondary antibody in chemiluminescent Western blotting applications.

本产品已经过优化,可作为二抗应用于化学发光的western blotting 中。


Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

化学发光已经成为western blots检测中最好和最全面的检测方法。它们减少了放射性物质和有毒显色基质相关的危害。这些方法的检测速度和灵敏度的都是传统替代品无可比拟的。因为结果生成在胶片上,因此可以永久记录和保存实验结果,而且用化学发光方法检测出的印迹可以很容易被洗脱并且易于采用其它抗体重新检测。辣根过氧化物酶(HRP)结合的二抗与化学发光的底物特异性结合生成光信号。辣根过氧化物酶与抗体的轭合物有非常高的周转率,在较短的时间里有很高的敏感性。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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