Product Pathways - TGF-beta/Smad Signaling
BMP6 (Precursor Specific) (D2V5Z) Rabbit mAb #70583
|70583S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|70583||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
BMP6 (Precursor Specific) (D2V5Z) Rabbit mAb recognizes endogenous levels of total precursor BMP6 protein. This antibody does not recognize mature BMP6 protein. This antibody also recognizes an unknown protein with a 26 kDa molecular weight.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln173 of human BMP6 protein.
Immunoprecipitation of BMP6 from A-204 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BMP6 (Precursor Specific) (D2V5Z) Rabbit mAb. Western blot analysis was performed using
BMP6 (Precursor Specific) (D2V5Z) Rabbit mAb and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678.
Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belong to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the C-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).
BMP6 plays a critical role in iron metabolism, ovulation, and aldosterone production (5-7). Recent studies have shown that hypermethylation of the BMP6 gene promoter decreases its expression in several cancer types, which contributes to tumor growth and progression (8-11).
- Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
- Wozney, J.M. et al. (1988) Science 242, 1528-34.
- Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
- Nohe, A. et al. (2004) Cell Signal 16, 291-9.
- Roth, M.P. and Coppin, H. (2009) Med Sci (Paris) 25, 678-80.
- Inagaki, K. et al. (2006) Endocrinology 147, 2681-9.
- Akiyama, I. et al. (2014) Reprod Sci 21, 772-7.
- Hashida, Y. et al. (2012) Oncol Rep 27, 825-30.
- Liu, G. et al. (2014) Oncol Rep 32, 581-8.
- He, Y. et al. (2014) PLoS One 9, e87994.
- Sangplod, P. et al. (2014) Asian Pac J Cancer Prev 15, 7091-5.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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