Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-ALK (Tyr1096) (D96H9) Rabbit mAb #6962

No. Size Price
6962S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
6962 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 (NPM-ALK) 220 (ALK) Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-ALK (Tyr1096) (D96H10) Rabbit mAb detects ALK only when phosphorylated at Tyr1096 (equivalent to Tyr156 of NPM-ALK). 磷酸化的ALK(Tyr1096)的兔源单克隆抗体(D96H10)仅在(Tyr1096)(对应NPM-ALK上156)被磷酸化后才能检测到ALK的存在。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1096 of human ALK protein. 单克隆抗体通过合成磷酸化多肽免疫动物得到,该多肽是根据人的ALK蛋白Tyr1096附近的氨基酸序列合成的。

Western blot analysis of extracts from SUP-M2 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1096) (D96H10) Rabbit mAb (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). 用抗磷酸化的ALK (Tyr1096) 的兔源单克隆抗体 (上) 或抗ALK的兔源单克隆抗体(C26G7) #3333(下)对经过或未经小牛肠碱性磷酸酶(CIP)处理的SUP-M2 细胞提取物进行蛋白质印记检测。


Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and EML4 has been described from a non-small cell lung cancer cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6,7).

间变性淋巴瘤激酶(ALK)是多效生长因子(PTN)的酪氨酸激酶受体,它是大脑胚胎发育过程中的生长因子(1-3)。 在表达ALK的细胞中,PTN诱导ALK及其下游效应因子IRS-1, Shc, PLCγ, 和PI3K激酶发生磷酸化(1)。ALK最初是从易位突变产生的NPM-ALK融合蛋白而被发现的(4)。NPM-ALK融合蛋白是一个组成性活化,有原癌基因活性的间变性淋巴瘤相关酪氨酸激酶(4)。 由NPM-ALK介导的PLCγ的活化可能对有丝分裂活性起到关键作用,并且可能对间变性淋巴瘤的发病过程很重要(5)。另一个完全不同的ALK原癌融合蛋白在非小细胞肺癌细胞株中被报道,该蛋白融合了ALK与EML4,其相应融合转录产物在一些肺腺癌中也有表达。在EML4-ALK融合蛋白中,微管相关蛋白EML4的短氨基末端区域和ALK的激酶活性区域融合在一起(6,7)。

Phosphorylation of ALK on Tyr1096 was identified at Cell Signaling Technology using PTMScan®, our LC-MS/MS platform for phosphorylation site discovery. Phosphorylation of fusion protein NPM-ALK at the Tyr1096 site was also reported by several other labs in select carcinoma cell lines and in tumors, and is shown to be important for NPM-ALK function (8,9).

ALK的Tyr1096位点磷酸化是Cell Signaling Technology (CST)公司利用LC-MS/MS平台PhosphoScan®技术在寻找磷酸化位点过程中发现的。NPM-ALK的Tyr1096位点磷酸化也被其他实验室报道在某些肿瘤及肿瘤细胞系中存在,并对NPM-ALK 的功能十分重要(8,9)。

  1. Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.
  2. Iwahara, T. et al. (1997) Oncogene 14, 439-49.
  3. Morris, S.W. et al. (1997) Oncogene 14, 2175-88.
  4. Morris, S.W. et al. (1994) Science 263, 1281-4.
  5. Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.
  6. Rikova, K. et al. (2007) Cell 131, 1190-203.
  7. Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.
  8. Soda, M. et al. (2007) Nature 448, 561-6.
  9. Turner, S.D. et al. (2007) Cell Signal 19, 740-7.
  10. Chikamori, M. et al. (2007) Oncogene 26, 2950-4.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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