Cell Signaling Technology

Product Pathways - Metabolism

NRF1 (D9K7R) Rabbit mAb #69432

Alpha palindromic-binding protein   Alpha-pal   NRF-1   NRF1   Nuclear respiratory factor 1  

No. Size Price
69432S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
69432 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 68 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

NRF1 (D9K7R) Rabbit mAb recognizes endogenous levels of total NRF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu278 of human NRF1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HepG2 and PC3 cell lysates using NRF1 (D9K7R) Rabbit mAb.

IP

IP

Immunoprecipitation of NRF1 from HepG2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NRF1 (D9K7R) Rabbit mAb. Western blot analysis was performed using NRF1 (D9K7R) Rabbit mAb.

Background

Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c (1). It was later found to play a role in the nuclear control of mitochondrial function (1). PGC-1 induces the expression of NRF1 and NRF2 (2). NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function (2).

  1. Virbasius, C.A. et al. (1993) Genes Dev 7, 2431-45.
  2. Wu, Z. et al. (1999) Cell 98, 115-24.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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