Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb #68790

No. Size Price
68790S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
68790 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 134 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb recognizes endogenous levels of Tyk2 protein only when phosphorylated at Tyr1054 and Tyr1055. Cross-reactivity was not observed with other Jak family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Tyr1054/1055 of human Tyk2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved U266 and Jurkat cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (50 ng/ml, 15 min; +) using Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb (upper) or total Tyk2 (D4I5T) Rabbit mAb #14193 (lower).

Background

Tyk2 is a member of the Jak family of protein tyrosine kinases. It associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and IFN-α and β (1-3). Following ligand binding, Tyk2 is activated by phosphorylation of Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-β signaling cascade (5).

The role of Tyk2 has been extensively studied in terms of its involvement in immune regulation and pathological significance (reviewed in 6). Deletion of Tyk2 in mice results in increased sensitivity to infection and defective tumor surveillance, but only a partial effect on Type I interferon signaling (7, 8). In contrast, a human patient diagnosed with hyper-IgE syndrome having increased susceptibility to various microorganisms was found to have a homozygous mutation of Tyk2 (9). These studies suggest a more critical role of Tyk2 in humans with regards to Type I interferon signaling as well as other cytokines including IL-23, IL-6, and IL-10.

  1. Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-34.
  2. Stahl, N. et al. (1994) Science 263, 92-5.
  3. Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  4. Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-500.
  5. Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-11.
  6. Strobl, B. et al. (2011) Front Biosci (Landmark Ed) 16, 3214-32.
  7. Karaghiosoff, M. et al. (2000) Immunity 13, 549-60.
  8. Shimoda, K. et al. (2000) Immunity 13, 561-71.
  9. Minegishi, Y. et al. (2006) Immunity 25, 745-55.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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