Cell Signaling Technology

Product Pathways - Protein Stability

VHL Antibody #68547

No. Size Price
68547S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
68547 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 18-22 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

VHL Antibody recognizes endogenous levels of total VHL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VHL protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VHL Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Note that 786-O is a VHL-null cell line, demonstrating specificity of the antibody.

Background

The Von Hippel-Lindau (VHL) protein is a substrate recognition component of an E3 ubiquitin ligase complex containing elongin BC (TCEB1 and TCEB2), cullin 1 (CUL1), and RING-box protein 1 (RBX1) (1,2,3). VHL protein has been shown to exist as three distinct isoforms resulting from alternatively spliced transcript variants (4). Loss of VHL protein function results in a dominantly inherited familial cancer syndrome that manifests as angiomas of the retina, hemangioblastomas of the central nervous system, renal clear-cell carcinomas, and pheochromocytomas (4). Under normoxic conditions, VHL directs the ubiquitylation and subsequent proteosomal degradation of the hypoxia inducible factor 1α (HIF-1α), maintaining very low levels of HIF-1α in the cell. Cellular exposure to hypoxic conditions, or loss of VHL protein function, results in increased HIF-1α protein levels and increased expression of HIF-induced gene products, many of which are angiogenesis factors such as vascular endothelial growth factor (VEGF). Thus, loss of VHL protein function is believed to contribute to the formation of highly vascular neoplasias (4). In addition to HIF-1α, VHL is known to regulate the ubiquitylation of several other proteins, including tat-binding protein 1 (TBP-1), the atypical protein kinase C lambda (aPKC), and two subunits of the multiprotein RNA Polymerase II complex (RPB1 and RPB7) (5,6,7,8). Interactions with elongin BC, RPB1, RPB7 and the pVHL-associated KRAB-A domain containing protein (VHLaK) suggest that VHL may also play a more direct role in transcriptional repression.

  1. Kibel, A. et al. (1995) Science 269, 1444-6.
  2. Pause, A. et al. (1997) Proc Natl Acad Sci U S A 94, 2156-61.
  3. Kamura, T. et al. (2000) Proc Natl Acad Sci U S A 97, 10430-5.
  4. Czyzyk-Krzeska, M.F. and Meller, J. (2004) Trends Mol Med 10, 146-9.
  5. Corn, P.G. et al. (2003) Nat Genet 35, 229-37.
  6. Na, X. et al. (2003) EMBO J 22, 4249-59.
  7. Kuznetsova, A.V. et al. (2003) Proc Natl Acad Sci U S A 100, 2706-11.
  8. Li, Z. et al. (2003) EMBO J 22, 1857-67.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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