Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Ch-TOG (D2Z8J) Rabbit mAb #67774

No. Size Price
67774S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
67774 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 225 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Ch-TOG (D2Z8J) Rabbit mAb recognizes endogenous levels of total ch-TOG protein. Based on the amino acid sequence of the peptide antigen, this antibody is expected to recognize all isoforms of ch-TOG.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala31 of human ch-TOG protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, transfected with control siRNA (-) or ch-TOG siRNA (+), using Ch-TOG (D2Z8J) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The Ch-TOG (D2Z8J) Rabbit mAb confirms silencing of ch-TOG expression, while the α-Actinin (D6F6) XP® Rabbit mAb is used as a loading control.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ch-TOG (D2Z8J) Rabbit mAb.

Background

Ch-TOG (colonic hepatic tumor overexpressed gene)/CKAP5 (cytoskeleton-associated protein 5) is a microtubule stabilizing protein involved in the organization of mitotic spindle poles through interaction with the transforming acid coiled-coil protein, TACC3 (1). Ch-TOG and TACC3 also interact with the membrane trafficking protein clathrin, and this interaction is thought to be required for clathrin’s mitotic function in crosslinking microtubules in the mitotic spindle (2). Researchers have found that expression levels of both TACC3 and ch-TOG are correlated with human diseases such as glioblastoma and hepatic carcinoma (3). A genome-wide siRNA screen identified ch-TOG and other G2/M phase regulators as potential contributors to head and neck squamous cell carcinoma (4).

  1. Gergely, F. et al. (2003) Genes Dev 17, 336-41.
  2. Royle, S.J. (2012) J Cell Sci 125, 19-28.
  3. Thakur, H.C. et al. (2013) Biol Chem 394, 1411-23.
  4. Martens-de Kemp, S.R. et al. (2013) Clin Cancer Res 19, 1994-2003.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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